Distance of suture from edge of tissue and horizontal distance between stitches affects mechanical strength of anastomosis. In this study, 180 bovine intestines were used to investigate the optimum pattern in 18 groups by considering 4, 6, and 8mm horizontal distance between stitches, and 3, 5, and 7mm distance from edge of tissue with a 3-0Silk and a 3- 0PDS sutures to maximize the strength of anastomosis (10 specimens in each group). Also, 80 specimens were used to investigate the maximum effective distance of suture from edge of tissue in 8 groups of: 3, 5, 7, and 10mm of distance from edge, with the same type of sutures. Tensile tests with an elongation rate of 5mm/min were performed for all groups. Based on the results, the pattern of 7-6 (distance from edge-distance between stitches) for both 3-0Silk and 3-0PDS, 5-6 and 7-4 for 3-0Silk, and 5-6 and 7-4 for 3-0PDS can be ordered as the best options among 18 different combinations. It was also found that increasing the distance from edge from 7mm to 10mm cannot cause a significant difference in mechanical strength. Results can help surgeons to improve the intestinal anastomosis and employed as an input for automatic suturing devices.